The conference is supported by the K 125561 (’Tuberculosis and Evolution’) research grant of the National Research, Development and Innovation Office (NKFIH - Hungary) & the Department of Biological Anthropology, University of Szeged, Hungary

A quarter of century after the first international ‘ICEPT’ meeting on the evolution of tuberculosis (1997, Szeged, Hungary) and ten years after ‘ICEPT-2’, ‘ICEPT-3’ – a new international TB evolution meeting – will be held in Szeged. This third conference will review the latest results of research on TB evolution and TB paleopathology again.

The main questions ofICEPT-3 are the same as previously:

• What is the oldest evidence of this infection on human and animal remains?;
• When did specific mutation(s) of the modern strains arise?;
• What was the relative importance of the different pathogenic species of the Mycobacterium genus in past populations?;
• Did the pre-contact American TB differ from the Old World infection?;
• How can we explore the dynamics of the host-pathogen co-evolution in the case of TB infection?;
• Can we reconstruct a consensual phylogeny of the Mycobacterium genus?;
• What do we know about the evolution of susceptibility/resistance patterns among human populations?;
• How can paleopathology and paleomicrobiology contribute to the research of TB evolution?;
• What are the main strategies to stop or moderate the re-emergence of TB?; and
• How can molecular phylogenetics contribute to the global fight against TB?.

The ICEPT-3 ‘Tuberculosis in Evolution’ Meeting will be held in a HYBRID format, allowing for both in-person and online participation.

The in-person conference venue will be the Institute of Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary: location

For online attendees, a Zoom link is provided for the
first day (5^th July 2022, Tuesday: link1; Meeting ID: 895 9832 4839; Passcode: 725973) and
second day (6^th July 2022, Wednesday: link2; Meeting ID: 834 9966 3729; Passcode: 291127) of the meeting.

Any person who wishes to attend the conference, in-person or online, is welcome and does not have to pay registration fees.

Because of the very tight conference schedule, the speakers are kindly asked to respect the 20-minute time limit (preferably, 15-minute talk and 5-minute discussion).

Similar to the ICEPT-2 conference proceedings (Szeged, Hungary, 2012; link), the ICEPT-3 conference proceedings will be published in a special issue of Tuberculosis.

VENUE: Institute of Biology, University of Szeged

Közép fasor 52, H-6726 Szeged, Hungary

Szent-Györgyi Albert Room

Moderators: Zsolt BERECZKI & László PAJA (University of Szeged)

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5^TH JULY 2022, TUESDAY

(ZOOM; Meeting ID: 895 9832 4839; Passcode: 725973)

1. ‘Greetings’ by Attila GÁCSER, head of the Institute of Biology, University of Szeged
2. ‘The ICEPID series’ by Olivier DUTOUR
3. ‘Three ICEPT meetings in Szeged’ by György PÁLFI

Albert ZINK, Heidi Y. JÄGER, Guido VALVERDE, Ildikó PAP, Ildikó SZIKOSSY, György PÁLFI, Frank MAIXNER: Tuberculosis in mummies – New findings, perspectives and limitations

Kara L. HOLLOWAY-KEW, Maciej HENNEBERG: Dynamics of tuberculosis infection in various populations during the 19^th and 20^th century: The impact of conservative and pharmaceutical treatments on epidemiological observations

Frank MAIXNER, Heidi Y. JÄGER, Mohamed S. SARHAN, Guido VALVERDE, Albert ZINK: Mycobacterium tuberculosis DNA extraction from mummified and skeletal human remains using Linear Polyacrylamide (LPA)

Heidi Y. JÄGER, Frank MAIXNER, Albert ZINK: A leap in the dark – How intra-bone variability of pathogen load affects metagenomic detection in ancient Mycobacterium tuberculosis DNA in skeletal human remains

Andrei SOFICARU, Mihai CONSTANTINESCU, Luminiţa ADREICA: Cases of tuberculosis from the St. Sava cemetery (Bucharest) and comments over a sample from the 20^th century

Ana Luisa SANTOS, Bruno M. MAGALHÃES, Cinzia ROGGIO: Changes in mortality by TB in non-industrialized Coimbra (Portugal): Trends between 1861 and 1914

Jenna M. DITTMAR, Piers D. MITCHELL, Sarah A. INSKIP, Craig CESSFORD, John E. ROBB: Diseased cities: An examination of tuberculosis and other chronic respiratory diseases surrounding the time of the Black Death in Cambridge, England

Françoise LE MORT, Oussama BAKER, Bérénice CHAMEL, Hélène COQUEUGNIOT, Olivier DUTOUR: Oldest evidence of tuberculosis in the Mediterranean islands: From the mainland to Cyprus

Anett GÉMES, Antónia MARCSIK, Katalin Éva GYENESEI, Tamás SZENICZEY, Krisztián KISS, Kitti KÖHLER, Tamás HAJDU: Tuberculosis in prehistory in Eastern Central Europe – Chronological and geographical distribution

Krisztián KISS, Antónia MARCSIK, Erika MOLNÁR, György PÁLFI, Mónika MERCZI, Ivett KŐVÁRI, Orsolya LÁSZLÓ, Sándor ÉVINGER, Csilla LIBOR, Katalin Éva GYENESEI, Csilla Emese NÉMETH, Ágota MADAI, Anett GÉMES, István JÁNOS, Tamás HAJDU, Tamás SZENICZEY: One millennium (2^nd–12^th century AD) of the White Plague in the Carpathian Basin – New cases, expanding knowledge

Csilla LIBOR, Tamás HAJDU, Loránd O. KOVÁCS, László KUNOS, Orsolya MATEOVICS-LÁSZLÓ: „TB on the spot” – Discussion on an atypical extra-spinal presentation of tuberculosis from a Late Medieval Hungarian cemetery

Olga SPEKKER, David R. HUNT, Erika MOLNÁR, György PÁLFI, Michael SCHULTZ: New insights into the palaeopathological diagnosis of tuberculous meningitis

Tanvi P. HONAP, Åshild J. VÅGENE, Kelly M. HARKINS, Michael S. ROSENBERG, Karen GIFFIN, Felipe CÁRDENAS-ARROYO, Laura Paloma LEGUIZAMÓN, Judith ARNETT, Jane E. BUIKSTRA, Alexander HERBIG, Johannes KRAUSE, Kirsten I. BOS, Anne C. STONE: Ancient Mycobacterium pinnipedii genomes recovered from non-coastal peoples from pre-contact era South America highlight the complexity of tuberculosis transmission in the past

Elizabeth A. NELSON, Aditya Kumar LANKALIPALLI, Maria SPYROU, Åshild VÅGENE, Susanna SABIN, Tiffiny A. TUNG, Alexander HERBIG, Kirsten I. BOS: Imperial decline, drought, and disease: Contextualizing tuberculosis in pre-Hispanic Peru

Kelly E. BLEVINS, Elizabeth A. NELSON, Alexander HERBIG, Johannes KRAUSE, Jane E. BUIKSTRA, Josefina MANSILLA LORY, Kirsten I. BOS, Anne C. STONE: Combining paleopathology and ancient DNA to infer the paleoepidemiology and evolutionary history of the Mycobacterium tuberculosis complex in pre-conquest Mesoamerica

Qingyun LIU: Evolutionary dynamics and local adaptation of Mycobacterium tuberculosis encountering different host populations

Jorge A. SUBY: Following the trail of tuberculosis in Argentina: From hunter-gatherers and pastoralists to industrialized populations

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6^TH JULY 2022, WEDNESDAY

(ZOOM; Meeting ID: 834 9966 3729; Passcode: 291127)

Roland BROSCH: Evolution of tubercle bacilli, from recombinogenic to clonal populations

Christophe SOLA, Gaëtan SENELLE, Muhammed Rabiu SAHAL, Kevin LA, Typhaine BILLARD-POMARES, Julie MARIN, Antoine BRIDIER-NAHMIAS, Guislaine REFRÉGIER, Etienne CARBONNELLE, Emmanuelle CAMBAU, Christophe GUYEUX: Mycobacterium tuberculosis genomics and Big Data

Christophe GUYEUX, Gaëtan SENELLE, Guislaine REFRÉGIER, Christophe SOLA: A deeper understanding of the evolutionary phylogeny of M. tuberculosis

Adrien LE MEUR, Rima ZEIN EDDINE, Christophe GUYEUX, Gaëtan SENELLE, Christophe SOLA, Fiona HAK, Charlotte GENESTET, Oana DUMITRESCU, Noémie BOZIER, Amaury PIERRARD, Stéphane SKOULOUBRIS, Hannu MYLLYKALLIO, Guislaine REFRÉGIER: The paradoxes of Mycobacterium tuberculosis molecular evolution and consequences for the inference of past molecular diversity

Olga SPEKKER, Orsolya Anna VÁRADI, András SZEKERES, Heidi Y. JÄGER, Albert ZINK, Margit BERNER, Doris PANY-KUCERA, Liesa STRONDL, Paul KLOSTERMANN, Levente SAMU, Kitty KIRÁLY, Zsolt BERECZKI, Erika MOLNÁR, György PÁLFI, Balázs TIHANYI: The first reported archaeological case with calvarial tuberculosis from the present-day territory of Hungary

Alexandra BUZHILOVA, Natalia BEREZINA: Tuberculosis in the daily life of the ancient Eastern European pastoralists (environmental aspects)

Ildikó PAP, Ildikó SZIKOSSY, György PÁLFI: 25 years of international TB research on the Vác Mummy Collection

Hélène COQUEUGNIOT, György PÁLFI, Bernard GÉLY, Olivier DUTOUR: Paleoimaging of Azilian human remains from Les Iboussières (France) as evidence of possible Upper Paleolithic tuberculosis

Olivier DUTOUR: The paleopathology and paleoepidemiology of Upper Paleolithic tuberculosis: Facts and theories

Igor MOKROUSOV: Pathobiology and phylogenomics of M. tuberculosis Beijing and LAM genotypes in Russia

Stefan NIEMANN: Using genomic approaches to understand drug resistance evolution of Mycobacterium tuberculosis

Matthias MERKER, Jean-Philippe RASIGADE, Maxime BARBIER, Helen COX, Silke FEUERRIEGEL, Thomas A. KOHL, Egor SHITIKOV, Kadri KLAOS, Cyril GAUDIN, Rudy ANTOINE, Roland DIEL, Sonia BORRELL, Sebastien GAGNEUX, Vladyslav NIKOLAYEVSKYY, Sönke ANDRES, Valeriu CRUDU, Ulrich NÜBEL, Philip SUPPLY, Stefan NIEMANN, Thierry WIRTH: Transcontinental spread and evolution of an MDR M. tuberculosis clade over five decades

Álvaro CHINER-OMS, Mariana Gabriela LÓPEZ, Miguel MORENO-MOLINA, Victoria FURIÓ, Iñaki COMAS: Gene evolutionary trajectories in Mycobacterium tuberculosis reveal temporal signs of selection

Thierry WIRTH, Emilie ROUSSEAU, Nour GHARBI, Matthias MERKER, Stefan NIEMANN: Tracking clock variations, contrasted demographies and selection signatures in Mycobacterium tuberculosis lineages: From simulations to the field

A brief tribute to David Minnikin’s oeuvre

Helen D. DONOGHUE: Tuberculosis in historical material using ancient DNA and cell wall lipids

Houdini H.T. WU, Oona Y-C. LEE, David E. MINNIKIN, Gurdyal S. BESRA: An era of mycobacterial cell wall lipid biomarkers

György PÁLFI, Olga SPEKKER, Erika MOLNÁR, Zsolt BERECZKI, Hélène COQUEUGNIOT, Olivier DUTOUR, Anna-marie TILLIER, Zsolt MESTER, Enikő SZVÁK, Wilfried ROSENDAHL, Antal SKLÁNITZ, Frank MAIXNER, Albert ZINK, David E. MINNIKIN, Ildikó PAP: Re-examination and possible TB infection of the Subalyuk Neanderthal remains (Subalyuk Cave, Hungary)

Oona Y-C. LEE, Houdini H.T. WU, David E. MINNIKIN, Gurdyal S. BESRA, Gareth LLEWELLYN, Christopher M. WILLIAMS, Heidi Y. JÄGER, Frank MAIXNER, Albert ZINK, Mihály GASPARIK, Ildikó PAP, György PÁLFI: Sensitive detection of mycobacterial cell wall lipid biomarkers in Neanderthal skeletons from Subalyuk, Hungary

Orsolya Anna VÁRADI, Olga SPEKKER, Heidi Y. JÄGER, Frank MAIXNER, Albert ZINK, Dávid RAKK, Csaba VÁGVÖLGYI, Gabriella TERHES, Enikő SZVÁK, Ildikó PAP, Ildikó SZIKOSSY, György PÁLFI, András SZEKERES, David E. MINNIKIN: The development, verification and objectives of a mycocerosic acid based HPLC-HRMS method for TB diagnostics in paleopathology